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KMID : 1094720150200050844
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 5 p.844 ~ p.848
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Modification of the dynamic nature of the chimeric fumarate two-component system in Escherichia coli via positive feedback loop
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Ganesh Irisappan
Maruthamuthu Murali Kannan
Yoo Ik-Keun
Hong Soon-Ho
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Abstract
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A positive feedback loop was introduced to modify the dynamic behavior of fumarate sensing DcuSZ chimera TCS. To construct the positive feedback loop, the ompR gene was cloned downstream of the ompC promoter. The ompC promoter induced the expression of OmpR, which in turn induced the expression of the ompC promoter. Through the introduction of this positive feedback loop, the transcriptional expression levels of ompC increased 2.6-fold. When GFP was used as a reporter protein, a 64% increase in fluorescence level was observed. These results suggest that sensitivity of the TCS based fumarate sensing system can be engineered through the introduction of a positive feedback loop.
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KEYWORD
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fumarate, OmpR, positive feedback loop, two-component system, Escherichia coli
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